Volume 9 Number 2 (Apr. 2019)
Home > Archive > 2019 > Volume 9 Number 2 (Apr. 2019) >
IJBBB 2019 Vol.9(2): 134-140 ISSN: 2010-3638
doi: 10.17706/ijbbb.2019.9.2.134-140

Synthesis and Characterization of Methylcellulose-Poly(ethylenimine)2k for Gene Delivery System

Hye Ji Lee, Ju Hyeon Jeon, Tae-il Kim
Abstract—In this study, methylcellulose-poly(ethylenimine)2k (MC-PEI2k) was synthesized for gene delivery system. First, MC was oxidized by periodate oxidation and PEI2k molecules were attached to oxidized MC via reductive amination reaction between aldehyde moieties of oxidized MC and amines of PEI2k. Chemical structures and molecular weights of MC-PEI2k were confirmed by 1H NMR and GPC, respectively. MC-PEI2k could condense pDNA into positively charged and nano-sized polyplex particles. MC-PEI2k showed concentration-dependent but lower cytotoxicity than PEI25k. Transfection efficiency of MC-PEI2k was found to be lower than PEI25k in HeLa cells in serum-free condition but higher in serum condition, due to its good serum-compatibility. These results suggested the potential of MC-PEI2k for gene delivery system.

Index Terms—Gene delivery, methylcellulose, poly(ethylenimine), serum-compatibility.

The authors are with Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea (email: seal1004@snu.ac.kr).

Cite: Hye Ji Lee, Ju Hyeon Jeon, Tae-il Kim, "Synthesis and Characterization of Methylcellulose-Poly(ethylenimine)2k for Gene Delivery System," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 9, no. 2, pp. 134-140, 2019.

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
  • Jun 10, 2019 News!

    The papers published in Vol. 9, No. 3 have all received dois from Crossref. [Click]

  • Jun 03, 2019 News!

    IJBBB Vol. 9, No. 3 has been published online!  [Click]

  • Apr 11, 2019 News!

    The papers published in Vol. 9, No. 2 have all received dois from Crossref. [Click]

  • Mar 08, 2019 News!

    IJBBB Vol. 9, No. 2 has been published online!  [Click]

  • Jan 11, 2019 News!

    The papers published in Vol. 9, No. 1 have all received dois from Crossref. [Click]

  • Read more>>