Abstract—Cold atmosphere plasma has been developed and applied as a novel technology for cancer treatment. In most researches cap was directly applied to irradiate cancer cells or tissues. In this study, we report that CAP plasma discharge in cell culture medium and studied its effects on melanoma cancer cells. CAP plasma treated cell culture medium was applied to melanoma and fibroblast cells. Cell viability test by using MTT assay showed that treatment of with CAP plasma had the strongest effect on inducing apoptosis to inhibit melanoma cell proliferation and further cell metastasis. The most important is that CAP plasma treatment do not have any harmful effect on normal fibroblast cell, but do have exhibited strong cell killing effect on B16/F10 melanoma cell.

Index Terms—Cold atmospheric plasma, non-invasive treatment, melanoma cell.

Yun-Ju Chuang and Pei-Ru Chen are with Department of Biomedical Engineering, Ming Chuan University, Taoyuan, Taiwan, R.O.C. (email: prchen@mail.mcu.edu.tw).
Ming-Chen Wang is with Department of Biomedical Engineering, Chung Yuan Christian University, Taoyuan, Taiwan, R.O.C.

Cite: Yun-Ju Chuang, Ming-Chen Wang, Pei-Ru Chen, "Effects of a Nonthermal Atmospheric Pressure Plasma Discharged in Cell Culture Medium on Melanoma Cell," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 9, no. 2, pp. 126-133, 2019.