Volume 11 Number 1 (Jan. 2021)
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IJBBB 2021 Vol.11(1): 1-7 ISSN: 2010-3638
DOI: 10.17706/IJBBB.2021.11.1.1-7

Effect of Glucose Supplementation and Shaking Speed on the Production of Bioactive Trastuzumab and Chimeric Anti-VEGF Antibody in the Cytoplasm of Escherichia coli

Phakti Charoenpun, Asawin Meechai, Dujduan Waraho-Zhmayev
Abstract— Therapeutic antibody market size has been dramatically increasing each year as new target antigens have been identified as well as new type of antibodies are being explored. They are superior than chemical drug as they are highly specific to their co gnate antigen thus can provide very effective medical treatment. Advances in antibody engineering allows for the development of the next-generation of therapeutic antibodies. The ability to produce antibodies in microorganism will further advance this development. Not only in terms of ease of manipulation but also in terms of production cost, which can help increasing accessibility to the patients. In previous study, we investigated the effect of temperature and induction level on the production of full-length humanized anti-Her2 and chimeric anti-VEGF IgGs expressed as cyclonal. In this study, we aim to improve the production yield by investigating the effect of glucose supplementation and shaking speed. When considering glucose addition, it was found that 25 g/L showed significantly improved relative solubility as well as full-length IgG yield for both IgGs with no obvious glucose toxicity. For the shaking speed, it was found that, shaking speed of 150 rpm was the most suitable condition as the relative solubility and relative full-length IgG achieved the highest values for both IgGs among the tested conditions. These conditions can be used as guidelines for fermentation in a bioreactor for further optimization.

Index Terms— Chimeric anti-VEGF, cyclonal, Escherichia coli shuffle T7 express, trastuzumab.

Phakti Charoenpun and Dujduan Waraho-Zhmayev are with Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand.
Asawin Meechai is with Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand; Department of Chemical Engineering, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand.

Cite: Phakti Charoenpun, Asawin Meechai, Dujduan Waraho-Zhmayev, " Effect of Glucose Supplementation and Shaking Speed on the Production of Bioactive Trastuzumab and Chimeric Anti-VEGF Antibody in the Cytoplasm of Escherichia coli," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 11, no. 1, pp. 1-7, 2021.


Copyright © 2021 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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