Abstract—Agave sisalana can be used as medicinal plant to treat some oxidative stress-caused diseases. This study aimed to fractionate and evaluate the antioxidant components of the ethanol extract from sisal waste. The crude ethanol extract of sisal waste was fractionated into petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and water fraction (WF). Among this different fractions, EAF had the strongest reducing power and radical scavenging capacity with IC₅₀ of 40 mg/mL against DPPH· and 870 mg/mL against OH·, respectively, comparable to or even lower than that of BHT. EAF also had the highest content of total phenolic (214.6 mgGAE/g) and total flavonoid (179.8 mgRE/g). HPLC-MS analysis revealed the major components of EAF were flavanols and flavanones compounds.

Index Terms—Antioxidant, free radicals scavenging, ethanol extract, sisal waste.

Sidan Luo, Zhaomei Wang, Die Hu are with School of Food Science & Engineering, South China University of Technology, Guangzhou, 510641, China (email: wangzm@scut.edu.cn).
Jian-Yong Wu is with Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Cite: Sidan Luo, Zhaomei Wang, Die Hu, Jian-Yong Wu, "Antioxidant Activity and Components of the Ethanol Extract of Sisal Waste," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 9, no. 4, pp. 248-257, 2019.