IJBBB 2019 Vol.9(4): 237-247 ISSN: 2010-3638
doi: 10.17706/ijbbb.2019.9.4.237-247
doi: 10.17706/ijbbb.2019.9.4.237-247
A Three-Layer Microfluidic Kidney Chip for Drug Nephrotoxicity Test
Lei Yin, Hongbo Zhang, Shih-Mo Yang, Wenjun Zhang
Abstract—Nowadays, in the preclinical and clinical studies of drugs, cell culture in petri dishes and animal
experiments still can’t adequately reflect the effects of new drugs on human kidneys, which highlights a
great need to develop a model with high accuracy to recapitulate organ function in vitro. Here, a three-layer
microfluidic kidney chip that can be used to mimic the structure and function of kidneys by integrating
polydimethyl siloxy (PDMS) microfluidic channels and porous membranes. We also developed a supporting
microfluidic culture platform for the long-term culture of kidney cells. On this basis, two types of cell lines
(renal proximal tubular epithelial cells, RPTECs, and peritubular capillary endothelial cells, PCECs) and
three types of drugs (ciplatin, DDP, gentamycin, GM, and cyclosporine A, CsA) were used for accurate
assessment of drug-induced nephrotoxicity. Considered of the effects of time, we also tested two dosing
regimens of GM. Cells were under the fluidic shear stress of continuous flow to mimic the pharmacokinetics
of single injection and continuous infusion. Unlike in petri dishes, cells cultured in the microfluidic kidney
chips had a better performance on both the cell growth and the drug nephrotoxicity evaluation. The
microfluidic devices presented here may be useful to mimic the renal tubular system in vitro for evaluating
drug nephrotoxicity during preclinical studies.
Index Terms—Drugs, nephrotoxicity, pharmacokinetics, vitro.
Lei Yin, Hongbo Zhang are with The School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai, PR China (email: hbzhang@ecust.edu.cn).
Shih-Mo Yang is with The Biomedical Science and Technology Research Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, PR China (e-mail: smyang@shu.edu.cn).
Wenjun Zhang is with The Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada.
Index Terms—Drugs, nephrotoxicity, pharmacokinetics, vitro.
Lei Yin, Hongbo Zhang are with The School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai, PR China (email: hbzhang@ecust.edu.cn).
Shih-Mo Yang is with The Biomedical Science and Technology Research Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, PR China (e-mail: smyang@shu.edu.cn).
Wenjun Zhang is with The Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada.
Cite: Lei Yin, Hongbo Zhang, Shih-Mo Yang, Wenjun Zhang, "A Three-Layer Microfluidic Kidney Chip for Drug Nephrotoxicity Test," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 9, no. 4, pp. 237-247, 2019.
General Information
ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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