Volume 4 Number 6 (Nov. 2014)
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IJBBB 2014 Vol.4(6): 483-489 ISSN: 2010-3638
doi: 10.17706/ijbbb.2014.4.6.483-489

The Effects of Steroid Hormones on Adipogenic Markers in 3T3-L1 Adipocytes

G. Alshammari, J. Brameld, M. Lomax
Abstract—Obesity is a crucial health issue, being a risk factor for the development of chronic metabolic diseases such as type 2 diabetes, hypertension, hyperlipidemia, cardiovascular diseases, and some types of cancer. Traditional methods to control obesity by decreasing food intake, increasing exercise, and medical interventions have not been successful and more fundamental knowledge at the level of genes is required. In men, low levels of testosterone have emerged as an important medical concern since testosterone deficiency is related to the development central obesity. White fat cells were grown in DMEM supplemented with 10% FBS. Lipid accumulation in 3T3-L1 adipocytes was assessed by staining for 1hr in a freshly diluted Oil Red O. RNA was extracted, cDNA synthesised and mRNA quantified using standard qRTPCR. 3T3-L1 treated with sex steroids (5, 15, 30 nM) for 13 days exhibited a non-significant decreased in adipogenic markers (Resistin and FABP 4) without clear change in Adipo Q expression. Conclusion Adipogenic markers were not affected by steroid hormones, and These data demonstrate the need to conduct more results to investigate the effect of sex steroid at different doses and  time courses especially during early stage of differentiation on transcription factors such as PPAR gamma 2, CEBP alpha, and CEBP betta.

Index Terms—Steroid hormones, obesity, chronic diseases.

Department of food science and Nutrition, King Saud University, Riyadh, Saudi Arabia (email: sbxga1@nottingham.ac.uk).
Molecular Nutrition Division, College of Bio-Sciences, the University of Nottingham, Nottinghamshire, UK.

Cite: G. Alshammari, J. Brameld, M. Lomax, "The Effects of Steroid Hormones on Adipogenic Markers in 3T3-L1 Adipocytes," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 6, pp. 483-489, 2014.

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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