Abstract—The compositions of the medium for recombinant Pichia pastoris producing omega-3 fatty acid were determined for maximum biomass production using crude glycerol as a carbon source and ammonium salts as nitrogen sources. The optimization of the medium was done using Box-Behnken a design software with 3 factors, 3 levels, 1 response for an optimal level of crude glycerol, (NH4)2SO4, (NH4)2HPO4. The optimum medium condition was 40 g/L crude glycerol, 3 g/L (NH4)2SO4, and 7 g/L (NH4)2HPO4. The highest biomass in flasks cultivation after 7 days production was 6.064 g/L DCW and 0.1516 g biomass/ g crude glycerol.

Index Terms—Pichia pastoris, recombinant omega-3 fatty acid, crude glycerol, Box-Behnken.

Le Thi Thuy Trinh, Mariena Ketudat-Cairns and Chotika Gosalawit are with School of Biotechnology, Institute of Agricultural of Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand. Apichat Boontawan is with School of Biotechnology, Institute of Agricultural of Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand; He is also with Center of Excellent in Agricultural Product Innovation, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.

Cite:Le Thi Thuy Trinh, Mariena Ketudat-Cairns, Chotika Gosalawit, Apichat Boontawan, "Optimization of Nitrogen Sources and Carbon Source for Recombinant Omega-3 Fatty Acid-Containing Biomass Production," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 11, no. 4, pp. 82-88, 2021.

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