Volume 10 Number 1 (Jan. 2020)
Home > Archive > 2020 > Volume 10 Number 1 (Jan. 2020) >
IJBBB 2020 Vol.10(1): 49-57 ISSN: 2010-3638
doi: 10.17706/ijbbb.2020.10.1.49-57

Application of the Synthetic Escherichia coli’s Twin-Arginine Translocation Pathway for the Detection of Intracellular Antibodies against Hepatitis C Viral Protease

Attapol Kamthong, Phenbunya Boonyalekha, Jeerapond Leelawattanachai, Dujduan Waraho-Zhmayev
Abstract—Hepatitis C is a liver disease caused by hepatitis C virus (HCV) infection. Protease inhibitor (PI) is included in the current oral direct-acting antiviral (DAA) combination therapy. However, these HCV PIs are small molecule drugs; therefore, they could have serious off-target adverse effects. New types of treatment such as antibody drugs that have very high specificity and selectivity would be a better alternative. Our study reports the application of the PROTECT (Protease inhibitor Recognition based On Tat Export after Cleavage Tampering) assay, an in vivo detection method for protease inhibiting intracellular antibodies (intrabodies) based on the bacterial twin-arginine translocation (Tat) pathway to the HCV NS3 protease system. This assay was designed such that when protease is co-expressed inside the bacterial cytoplasm, Tat transportation of the uncleaved protease substrate due to protection of the cleavage site by a specific intrabody will result in β-lactam antibiotic resistance. Using the anti-NS3 intrabodies isolated previously, we demonstrated that PROTECT assay could distinguish between the inhibitory and non-inhibitory anti-NS3 intrabodies resulting in selective growth in the presence of β-lactam antibiotics. This method has potential for the screening of agents that inhibit proteolytic cleavage in a bacterial cell-based assay, which may find use in identifying, reconstituting, and characterizing protease inhibitors, identifying mutations on the substrate that can inhibit proteolytic cleavage, and drug screening.

Index TermsEscherichia coli, intracellular antibodies, protease inhibitors, twin-arginine translocation pathway.

Attapol Kamthong, Phenbunya Boonyalekha, Dujduan Waraho-Zhmayev are with Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand (email: dujduan.war@mail.kmutt.ac.th).
Jeerapond Leelawattanachai is with ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.

Cite: Attapol Kamthong, Phenbunya Boonyalekha, Jeerapond Leelawattanachai, Dujduan Waraho-Zhmayev, "Application of the Synthetic Escherichia coli’s Twin-Arginine Translocation Pathway for the Detection of Intracellular Antibodies against Hepatitis C Viral Protease," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 10, no. 1, pp. 49-57, 2020.


Copyright © 2020 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
  • Sep 29, 2022 News!

    IJBBB Vol 12, No 4 has been published online! [Click]

  • Jun 23, 2022 News!

    News | IJBBB Vol 12, No 3 has been published online! [Click]

  • Dec 20, 2021 News!

    IJBBB Vol 12, No 1 has been published online!  [Click]

  • Sep 23, 2021 News!

    IJBBB Vol 11, No 4 has been published online! [Click]

  • Jun 25, 2021 News!

    IJBBB Vol 11, No 3 has been published online! [Click]

  • Read more>>