Abstract—Biochemists and cell biologists use 1D gel electrophoresis to analyze protein compositions in samples. PAGE, a.k.a. sodium-dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis, uses electric current to separate proteins. The proteins are visualized by staining or immunofluorescent techniques. The result of a gel is captured in an image which is usually analyzed by visual inspection. As the banding patterns get more complex and the amount of differential intensity of bands on a gel increases, an elaborate methodology is required for the analysis. For systematic processing and analyzing complex gels, we have developed new techniques for recognition, assessment and quantitative interpretation in bands on individual lanes. In this manner, we compare similarities and differences between lanes of a gel. Specific attention is given on the features that are characterizing the banding pattern. It is important to make sure all lanes of gels in an experiment can be unambiguously assessed. Therefore, lane normalization is applied after background correction. With this regard, groups of gels within an experiment can be quantitative assessed. Automation will further speed up to the process of interpretation that can now be accomplished by objective means.

Index Terms—1D electrophoresis, PAGE gel, pattern extraction, feature extraction, image analysis.

F. Cai and F. J. Verbeek are with Section Imaging and Bioinformatics, LIACS, Leiden University, Leiden, Netherlands (email: f.j.verbeek@liacs.leidenuniv.nl).
S. Liu, P. Ten Dijke are with Department of Molecular Cell Biology, LUMC, Leiden University, Leiden, Netherlands.

Cite: F. Cai, S. Liu, P. Ten Dijke, F. J. Verbeek, "Image Analysis and Pattern Extraction of Proteins Classes from One-Dimensional Gels Electrophoresis," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 7, no. 4, pp. 201-212, 2017.