Volume 6 Number 4 (Oct. 2016)
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IJBBB 2016 Vol.6(4): 139-144 ISSN: 2010-3638
doi: 10.17706/ijbbb.2016.6.4.139-144

Genetic Diversity of Plain Medium Resistant of Chrysanthemum Planlet Produced of Gamma Ray Irradiation

Ari Wijayani, Rina Srilestari
Abstract—Genetic diversity can be improved using the gamma ray irradiation to obtain the resistant plants to the disease. The Changes occur can be analyzed at the beginning of plant growth using molecular analysis; RAPD technique (Random Amplified Polymorfism DNA). This study aims to determine the genetic diversity produced by gamma rays irradiated and given the poly ethilene glycol given in plantlets to increase chrysanthemum resistance to Medium plain. The study used four primers tested on four treatments that were not irradiated plantlets and induced the poly ethilene glycol, gamma rays irradiated 25 gray un induced poly ethilene glycol, irradiated and induced the poly ethilene glycol 15 and 30%.The results showed a decrease of control diversity of genetic 0.20 to 0.30 in 25 gray irradiation treatment with given the poly ethilene glycol 15%.The use of the RAPD technique to determine genetic diversity resulting from gamma ray irradiation on the level of plantlets. Gamma ray irradiation treatment causing mutations in all treatments tested. Plantlets of 25 gray irradiation induced with 15%poly ethylene glycol / l showed changes in DNA structure.

Index Terms—Chrysanthemum, irradiation, polyethylene-glycol.

The authors are with Department of Agrotechnology, Faculty of Agriculture, UPN “Veteran” Yogyakarta, Indonesia (e-mail: ariewijayani@yahoo.com).

Cite: Ari Wijayani, Rina Srilestari, "Genetic Diversity of Plain Medium Resistant of Chrysanthemum Planlet Produced of Gamma Ray Irradiation," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 6, no. 4, pp. 139-144, 2016.

General Information

ISSN: 2010-3638
Frequency: Bimonthly (2011-2015); Quarterly (Since 2016)
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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