Volume 5 Number 4 (July 2015)
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IJBBB 2015 Vol.5(4): 256-263 ISSN: 2010-3638
doi: 10.17706/ijbbb.2015.5.4.256-263

Isolation and Characterization of Wolbachia (Rickettsiales: Rickettsiaceace) from Several Economic Importance Parasitoids (Hymenoptera: Braconidae)

Muhamad Azmi Mohammed, Ameyra Aman Zuki, Suhana Yusof, Salmah Yaakop
Abstract—Endosymbiotic relationship between Wolbachia (Rickettsiales: Rickettsiaceace) and braconids species is a new topic to be discussed among researchers due to its potential as biocontrol agents of fruit flies species. In this study, Wolbachia Surface Protien (wsp) marker has been used to detect the existence of Wolbachia molecularly that associated with braconids (Opiinae) collected from several localities throughout Peninsular Malaysia Our results revealed that five species of parasitoids viz. Fopius arisanus, F. vandensboschi, Psyttalia sp., P. fletcheri and P. incisi were symbiont positively with nine different strains of Wolbachia. A total of nine strains have been successfully sequenced and classified into three Wolbachia subgroups namely Mel, Ri, and Inc based on the results of clustering analysis. In this study, Inc Wolbachia group has been known as a novel group emerged of Wolbachia. These data should be useful in future Wolbachia-based biological control program that involving parasitoids and fruit flies.

Index Terms—Endosymbiont, Wolbachia, parasitoids, braconidae, opiinae.

Centre for Insects Systematic, School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Horticulture Research Centre, Malaysian Agriculture for Research and Development Institute (MARDI), Persiaran MARDI-UPM, Ibu Pejabat MARDI, 43400 Serdang, Selangor, Malaysia.

Cite: Muhamad Azmi Mohammed, Ameyra Aman Zuki, Suhana Yusof, Salmah Yaakop, "Isolation and Characterization of Wolbachia (Rickettsiales: Rickettsiaceace) from Several Economic Importance Parasitoids (Hymenoptera: Braconidae)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 5, no. 4, pp. 256-263, 2015.

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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