Volume 5 Number 4 (July 2015)
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IJBBB 2015 Vol.5(4): 241-248 ISSN: 2010-3638
doi: 10.17706/ijbbb.2015.5.4.241-248

Development of Fluorescent Phycocyanin-Cu2+ Chemosensor for Detection of Homocysteine

Premsak Puangploy, Sukunya Oaew, Werasak Surareungchai
Abstract—A simple optical chemosensor from phycocyanin (L1), a protein-pigment complex extracted from cyanobacterium Spirulina platensis, has been developed. The L1 shows high selectivity toward Cu2+ by exhibiting spectral shift and quenching of fluorescence intensity. This L1-Cu2+ complex, so called ensemble probe, can be used to detect mercapto biomolecules, and has superior affinity toward homocysteine (Hcy). Upon binding to mercapto biomolecules, the color change from colorless-to-blue and the fluorescence emission change from colorless to pink were observed. This chemosensor could detect Hcy at concentration as low as 4.71 μM (S/N = 3) with a linear range of 10-100 μM (R2 = 0.9903). This sensing approach may have found applications in clinical diagnostics.

Index Terms—Phycocyanin, copper (II) ion, mercapto biomolecules, homocysteine, turn-on fluorescence.

School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkhuntien- Chaitalay Road, Thakam, Bangkok 10150, Thailand.
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.

Cite: Premsak Puangploy, Sukunya Oaew, Werasak Surareungchai, "Development of Fluorescent Phycocyanin-Cu2+ Chemosensor for Detection of Homocysteine," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 5, no. 4, pp. 241-248, 2015.

General Information

ISSN: 2010-3638
Frequency: Bimonthly (2011-2015); Quarterly (Since 2016)
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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