Volume 3 Number 5 (Sep. 2013)
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IJBBB 2013 Vol.3(5): 513-515 ISSN: 2010-3638
DOI: 10.7763/IJBBB.2013.V3.267

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

M. Siti Hatijah and W. R. Wan Ruhayu
Abstract—Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8), the sucrose concentration (10g/l to 50 g/l) and the agitation rate (100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30oC. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration.

Index TermsAspergillus flavus, gout, uric acid, uricase.

M. Siti Hatijah and W. R. Wan Ruhayu are with the Faculty of Chemical Engineering & Natural Resources, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia (e-mail: hatijah@ump.edu.my).

Cite:M. Siti Hatijah and W. R. Wan Ruhayu, "Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 5, pp. 513-515, 2013.

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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