Abstract—Candida albicans is a most common fungal pathogen that causes oral and genital infections in human. This pathogen can causes candidiasis, especially in person with immunocompromised. Local isolated C. albicans has been successfully identified based on its phenotype and genotype characteristics. ITS amplification was performed using ITS1 and ITS4 primers. C. albicans is also exhibits as mesophilic fungus. Due to this reason, we believed that this microorganism has the potential to produce a mesophilic form of DNA helicase. In this study, a potential DNA repair helicase gene has been successfully amplified and identified from the local isolated C. albicans. DNA helicase was known as a motor protein for unwinding of duplex DNA into single stranded DNA using energy derived from ATP hydrolysis. However, a further study is needed to verify and validate the DNA helicase unwinding activity in future.

Index Terms—Candida albicans, DNA helicase, genotype, mesophilic fungus.

Haw Boon Ping, Eugene Ong Boon Beng and Asma Ismail are with the Institute for Research in Molecular Medicine (INFORMM), USM, 11800 USM, Penang, Malaysia (e-mail: hbping87@gmail.com, srisasidharan@yahoo.com, asmainformm @ yahoo.com, eugene@usm.my).

 

Cite:Haw Boon Ping, Asma Ismail, Eugene Ong Boon Beng, and Sasidharan Sreenivasan, "Isolation and Identification of Potential DNA Helicase from Local Isolate of Candida Albicans," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 5, pp. 505-508, 2013.