Abstract—Colon cancer is one of the leading causes of cancer-associated morbidity and mortality worldwide. The development of colon cancer is closely related to epidermal growth factor receptor (EGFR) pathway. Son of Sevenless Homolog 1 (SOS1) gene is a key component in the EGFR pathway that has been reported to be overexpressed in cancer. The aim of the study was to investigate the microRNAs that potentially regulate SOS1 expression in colon cancer patients. A total of 60 cancerous and adjacent non-cancerous tissues were collected. Western blot, microRNA microarray and quantitative real-time PCR analyses were carried out. Significant overexpression of SOS1 and downregulation of miR-195 were determined (p < 0.05). The findings suggested a potential regulation of SOS1 expression by miR-195.

Index Terms—Colon cancer, microRNA, SOS1, TNM.

Fung Lin Yong and Chee Wei Law are with Department of Surgery, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia (e-mail: flyong88@yahoo.com, drcwlaw@gmail.com).
Chee Woon Wang is with Department of Biochemistry, Faculty of Medicine, MAHSA University College, 59100 Kuala Lumpur, Malaysia (e-mail: wang.chee@mahsa.edu.my).

 

Cite:Fung Lin Yong, Chee Wei Law, and Chee Woon Wang, "MicroRNAs that Potentially Regulate SOS1 Expression in Colon Cancer," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 5, pp. 424-428, 2013.