Abstract—Powdery mildewis one of the destructive diseases of wheat in many regions of the world. Wheat-Thinopyrumintermedium derived line CH5382 conferring novel powdery mildew resistance was characterized using molecular and cytogenetic methods. The conventional GISH analysis probed by Th. Intermedium cannot detect the alien fragmentof CH5382. Two PCR-based Landmark Unique Gene markers (TNAC1102 and TNAC1567), which were assigned on wheat chromosome 2L and 5S, respectively, canamplfy unique bands of CH5382, and the bands were traced to wheat-Th. intermedium partial amphiploiddonor TAI7044 and Th. intermedium. The result suggested that CH5382 is a new wheat- Thinopyrumintermedium cryptic alien translocation line with powdery mildew resistance.

Index Terms—Cryptic alientranslocation, landmark unique gene markers, powdery mildew, Thinopyrumintermedium.

H. X. Zhan, Z. J. Yang and G. R. Li are with the School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, China (e-mail: zhan030006@126.com, ligr28@126.com, yangzujun@uestc.edu.cn).
Z.J. Chang, X. J. Zhang, X. li and H. J. Guo are with the Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China (e-mail: wrczj@126.com, zxjemail@163.com, 52518178@qq.com, 287145025@qq.com).

 

Cite:Hai-Xian Zhan, Guang-Rong Li, Zhi-Jian Chang, Xiao-Jun Zhang, Xin Li, and Zu-Jun Yang, "Molecular Identification of a Newwheat-Thinopyrumintermedium Cryptictranslocation Line for Resistance to Powdery Mildew," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 4, pp. 376-378, 2013.