Volume 3 Number 4 (Jul. 2013)
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IJBBB 2013 Vol.3(4): 363-367 ISSN: 2010-3638
DOI: 10.7763/IJBBB.2013.V3.233

Analyzing of Major Active Luteolin in Vernonia Cinerea

Guan-Rui Lai and Chiao-Song Wu
Abstract—This study investigates the extraction of the Vernonia cinerea using supercritical carbon dioxide extraction under the following conditions pressure at 300, 400 and 500 bar, temperatures at 313, 323 and 333 K, CO2 flow rate at 2 and 3 L/min. The best operation of extraction is under 500 bar, 333 K and 3 L/min CO2 flow rate. The reverse-phase high performance liquid chromatography method was developed for determining major active components of Vernonia cinerea. We dissolve the sample with methanol and analyzed by using a MetaChem C18-A(250 × 4.6m, 5μ) column with a gradient elution composed of ultra-pure water and acetonitrile. The wavelength of UV detector was set at 280 nm and the flow-rate was set at 1 mL/min. Under this condition,

Index Terms—Vernonia cinerea, supercritical carbon dioxide, luteolin, flavonoids.

Guan-Rui Lai is with Ming Chi University of Technology, Department of Chemical Engineering, TW (e-mail: qooforme@yahoo.com.tw).

Cite:Guan-Rui Lai and Chiao-Song Wu, "Analyzing of Major Active Luteolin in Vernonia Cinerea," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 4, pp. 363-367, 2013.

General Information

ISSN: 2010-3638
Frequency: Bimonthly (2011-2015); Quarterly (Since 2016)
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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