Abstract—In this study, two Tenera breeds were used in the preparation of Palm Puree (PP), namely T24 and T99. Each breed was formulated with two different PP formulations which made of 2% mesocarp fibre with 98% crude palm oil (labeled as A) and 5% mesocarp fiber with 95% crude palm oil (labeled as B). The four formulations were named as PP24A, PP24B, PP99A and PP99B. Total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity of four Palm Puree formulations were determined using spectrophotometric methods. Identification and quantification of individual polyphenolic acid compounds were performed using reversed phase high performance liquid chromatography. TPC of all samples varied from 486.33 to 778.29 mg GAE/100 g EW. The amount of TFC ranged between 30.08 and 52.01 mg CE/100 g EW. The FRAP value varied from 585.58 to 1234.06 mM TE/g EW. While for the IC50 ranged between 100.24 and 156.38 μg/ml. A strong correlation was found between the TPC and the antioxidant capacity, indicating that polyphenolic acid would be responsible in scavenging the free radicals. Syringic acid was found to be the most predominant phenolic acid in all formulations.

Index Terms—Antioxidant, crude palm oil, mesocarp fibre, palm puree.

M. Haswani Maisarah, A. Noriham, and M. N. Zainon are with the Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia (e-mail: haswani.maisarah@yahoo.com, noriham985@salam.uitm.edu.my, dzainonmn@salam.uitm.edu.my).

 

Cite:M. Haswani Maisarah, A. Noriham, and M. N. Zainon, "Quantification of Polyphenolic Acids and Antioxidant Capacity of Palm Puree from Different Tenera Breeds of Elaeis Guineensis," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 4, pp. 349-353, 2013.