Volume 3 Number 1 (Jan. 2013)
Home > Archive > 2013 > Volume 3 Number 1 (Jan. 2013) >
IJBBB 2013 Vol.3(1): 37-42 ISSN: 2010-3638
DOI: 10.7763/IJBBB.2013.V3.159

Mutagenesis of a Copper P-Type ATPase Encoding Gene in Methylococcus capsulatus (Bath) Results in Copper-Resistance

Ashraf Y. Z. Khalifa
Abstract—Copper is an essential micronutrient for all living cells, however, it is extremely toxic at high concentrations and thus copper homeostasis is required to be tightly regulated. copA encodes for a copper-translocating P-type ATPase (CopA) and plays a vital role in copper homeostasis and is involved in copper transport across membranes of many organisms. Little is known about copper homeostasis in Methylococcus capsulatus although copper has a significant physiological role in this methanotroph. In this study we investigated the disruption of a CopA1 homologue (MCA2072; copA1) in M. capsulatus (Bath) by insertional inactivation mutagenesis. The resulting mutant, M. capsulatus ΔcopA1, was copper resistant to elevated copper concentrations (100 μM) than the wild-type strain (80 μM). Furthermore, the intracellular copper measurements revealed that ΔcopA1 accumulated half the amount of copper when compared with the wild-type. No observed phenotypic difference between the mutant strain and wild-type related to growth at different silver concentrations. These observations suggest that M. capsulatus CopA1 has a key role in copper homeostasis.

Index Terms—CopA, copper homeostasis, P-type ATPases, Methylococcus capsulatus

Ashraf Y. Z. Khalifa is with Botany Department, University of Beni-Suef, Beni-Suef, Egypt (e-mail: ashraf_zaky2002@yahoo.com)

Cite:Ashraf Y. Z. Khalifa, "Mutagenesis of a Copper P-Type ATPase Encoding Gene in Methylococcus capsulatus (Bath) Results in Copper-Resistance," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 2, pp. 37-42, 2013.

General Information

ISSN: 2010-3638
Frequency: Bimonthly (2011-2015); Quarterly (Since 2016)
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
  • Jan 05, 2017 News!

    [CFP] 2017 the annual meeting of IJBBB Editorial Board, ICOCB 2017, will be held in Jakarta, Indonesia during May 24-26, 2017. [Click]

  • Sep 12, 2017 News!

    The papers published in Vol. 7, No. 4 have all received dois from Crossref. [Click]

  • Aug 29, 2017 News!

    IJBBB Vol. 7, No. 4 has been published online!  [Click]

  • Jun 12, 2017 News!

    The papers published in Vol. 7, No. 3 have all received dois from Crossref. [Click]

  • May 19, 2017 News!

    IJBBB Vol. 7, No. 3 has been published online!  [Click]

  • Read more>>