Volume 2 Number 5 (Sep. 2012)
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IJBBB 2012 Vol.2(5): 309-313 ISSN: 2010-3638
DOI: 10.7763/IJBBB.2012.V2.123

Cost-Effective Expression and Purification of Recombinant Venom Peptide Mastoparan B of Vespa Basalis in Escherichia Coli

Chi-Cung Peng, Jia-Yang Hong, and Wu-Chun Tu

Abstract—Mastoparan B is a cationic, amphiphilic tetradecapeptide toxin isolated from the venom of the black-bellied hornet (Vespa basalis), the most dangerous species of wasps found in Taiwan. Mastoparan B was evaluate possess antibacterial activity and cardiovascular depress activity. However, mastoparan B is low abundance in the venom (3.4% of crude venom). In this study, mastoparan B was overexpressed in Escherichia coli BL21 as a recombinant protein fused the C-terminus of oleosin by a linker polypeptide, intein S. Artifical oil bodies (AOBs) were reconstituted with triacylglycerol, phospholipid to obtain the insoluble recombinant protein. Mastoparan B was subsequently released through self-splicing of intein induced by temperature alteration from artifical oli bodies, and the recombinant mastoparan B was collected it in the supernatant after centrifugation. Recombinant mastoparan B release from AOBs was exhibited membrane permeabilization activity, bacteriostatic and bactericidal activity. These results have shown that mastoparan B was successfully expressed and purified via the efficient AOB expression/purification system.

Index Terms—Vespa basalis, mastoparan B, artifical oil bodies (AOBs) expression/puruification system, bacteriostatic and bactericidal activity.

C. C. Peng is with the Department of Biotechnology, National Formosa University, Taiwan
J. Y. Hong is with the Graduate Institute of Electro-Optical and Materials Science, National Formosa University, Taiwan
W. C. Tu is with the Department of Entomology, National Chung-Hsing University, Taiwan (e- mail: bocky@sunws.nfu.edu.tw).

Cite: Chi-Cung Peng, Jia-Yang Hong, and Wu-Chun Tu, "Cost-Effective Expression and Purification of Recombinant Venom Peptide Mastoparan B of Vespa Basalis in Escherichia Coli," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 5, pp. 309-313, 2012.

General Information

ISSN: 2010-3638
Frequency: Bimonthly (2011-2015); Quarterly (Since 2016)
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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