Volume 11 Number 4 (Oct. 2021)
Home > Archive > 2021 > Volume 11 Number 4 (Oct. 2021) >
IJBBB 2021 Vol.11(4): 82-88 ISSN: 2010-3638
DOI: 10.17706/IJBBB.2021.11.4.82-88

Optimization of Nitrogen Sources and Carbon Source for Recombinant Omega-3 Fatty Acid-Containing Biomass Production

Le Thi Thuy Trinh, Mariena Ketudat-Cairns, Chotika Gosalawit, Apichat Boontawan
Abstract—The compositions of the medium for recombinant Pichia pastoris producing omega-3 fatty acid were determined for maximum biomass production using crude glycerol as a carbon source and ammonium salts as nitrogen sources. The optimization of the medium was done using Box-Behnken a design software with 3 factors, 3 levels, 1 response for an optimal level of crude glycerol, (NH4)2SO4, (NH4)2HPO4. The optimum medium condition was 40 g/L crude glycerol, 3 g/L (NH4)2SO4, and 7 g/L (NH4)2HPO4. The highest biomass in flasks cultivation after 7 days production was 6.064 g/L DCW and 0.1516 g biomass/ g crude glycerol.

Index Terms—Pichia pastoris, recombinant omega-3 fatty acid, crude glycerol, Box-Behnken.

Le Thi Thuy Trinh, Mariena Ketudat-Cairns and Chotika Gosalawit are with School of Biotechnology, Institute of Agricultural of Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand. Apichat Boontawan is with School of Biotechnology, Institute of Agricultural of Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand; He is also with Center of Excellent in Agricultural Product Innovation, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.

Cite:Le Thi Thuy Trinh, Mariena Ketudat-Cairns, Chotika Gosalawit, Apichat Boontawan, "Optimization of Nitrogen Sources and Carbon Source for Recombinant Omega-3 Fatty Acid-Containing Biomass Production," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 11, no. 4, pp. 82-88, 2021.

Copyright © 2021 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

General Information

ISSN: 2010-3638 (Online)
Abbreviated Title: Int. J. Biosci. Biochem. Bioinform.
Frequency: Quarterly 
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing:  Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
  • Sep 23, 2021 News!

    IJBBB Vol 11, No 4 has been published online! [Click]

  • Jun 25, 2021 News!

    IJBBB Vol 11, No 3 has been published online! [Click]

  • Mar 29, 2021 News!

    IJBBB Vol 11, No 2 has been published online!  [Click]

  • Dec 03, 2020 News!

    IJBBB Vol 11, No 1  has been published online! [Click]

  • Sep 28, 2020 News!

    IJBBB Vol 10, No 4 has been published online! [Click]

  • Read more>>