Volume 5 Number 6 (Nov. 2015)
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IJBBB 2015 Vol.5(6): 329-335 ISSN: 2010-3638
doi: 10.17706/ijbbb.2015.5.6.329-335

Rapid Detection of Foodborne Pathogens by Surface Plasmon Resonance Biosensors

Esma Eser, Okan Öner Ekiz, Hikmet Çelik, Selim Sülek, Aykutlu Dana,H. İbrahim Ekiz
Abstract—Here, we report rapid and sensitive detection of Salmonella Enteritidis with high specificity by using SPR (Surface Plasmon resonance) technique. Antibodies against S. Enteritidis were immobilized on to a gold sensor surface and different concentrations of serially diluted S. Enteritidis solutions were pumped in to the system. The changes in the response unit (RU) due to the binding of different concentrations of bacteria were measured. Regeneration of the sensor surface was carried out by using 0.05 % PBS + Tween 80 solution. The selectivity of the developed sensor was examined with Listeria monocytogenes which did not produce any significant response. Additionally, the ability of the developed sensor to detect S. Enteritidis in milk samples was tested. Based on these results, the developed SPR based biosensor may be used for rapid, label-free, sensitive and selective detection of bacteria in food materials.

Index Terms—Surface Plasmon Resonance (SPR), biosensors, Salmonella Enteritidis, Listeria monocytogenes, milk.

Esma Eser, H. İbrahim Ekiz are with the Department of Food Engineering, Mersin University, 33343 Mersin, Turkey.
Okan Öner Ekiz, Hikmet Çelik, Selim Sülek are with the Nanodev Scientific, Bilkent Cyberpark, Cyberplaza Vakıf Binası, B01-M13, 06800 Ankara, Turkey.
Okan Öner Ekiz, Aykutlu Dana are with the UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800, Ankara, Turkey.

Cite: Esma Eser, Okan Öner Ekiz, Hikmet Çelik, Selim Sülek, Aykutlu Dana,H. İbrahim Ekiz, "Rapid Detection of Foodborne Pathogens by Surface Plasmon Resonance Biosensors," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 5, no. 6, pp. 329-335, 2015.

General Information

ISSN: 2010-3638
Frequency: Bimonthly (2011-2015); Quarterly (Since 2016)
DOI: 10.17706/IJBBB
Editor-in-Chief: Prof. Ebtisam Heikal 
Abstracting/ Indexing: Electronic Journals Library, Chemical Abstracts Services (CAS), Engineering & Technology Digital Library, Google Scholar, and ProQuest.
E-mail: ijbbb@iap.org
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