Abstract—Benzimidazole is an adsorption reagent and popularly used in production of anthelmintic, antifungal and antibacterial drugs. Benzimidazole is chemical reagent including of heterocyclic aromatic. In this work, citronellal was used as the starting material which was extracted from Citrus hystrix (makrut lime) leaves that contains aldehyde functional group. The essential oil was reacted with 1,2-phenylene diamine via condensation reaction in ethanol to give benzimidazole derivative. The synthesized derivatives are characterized by fourier transform infrared spectroscopy (FT-IR) that exhibited the spectrum (N-H str) at 3389 cm-1, (C-N str) 1272 cm-1, (C=C str) of benzene ring at 1655 and 1460 cm-1and (C=C str) of alkene at 1379 cm-1 respectively which are a functional group of benzimidazole. Moreover, when compared between the IR spectrum of product compound and the substrate (citronellal), it was found that the character peak of aldehyde (C=O) was missed due with the reaction. In addition to these product was tested the antibacterial activity with Gram negative, Escherichia coli (E. coli), and Gram positive, Staphylococcus Aureus , bacterial strains by disc-diffusion methods at a concentration of 200mg/ml, using ethanol as the control, compare with antibacterial drug, Gentamicin. These result suggest that the derivative effective excellent antibacterial against than antibacterial drug. It may be the medicine to prevent and treat many bacterial diseases.

Index Terms—Benzimidazole, citronellal, lime leaves

The authors are with Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, 50290, Thailand

Cite: Uthumporn Kankeaw, Ratchaneeporn Rawanna, "The Study of Antibacterial Activity of Benzimidazole Derivative Synthesized from Citronellal," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 5, no. 5, pp. 280-287, 2015.